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American Society of Microbiology ABMM Sample Questions:

1. A public health investigation is underway following several cases of encephalitis in a specific geographic region during the summer months. Mosquitoes are abundant in the area. Serological testing of patient CSF samples reveals IgM antibodies against a flavivirus. The MOST likely causative agent is:

A) Jamestown Canyon virus
B) West Nile virus
C) Rabies virus
D) Eastern equine encephalitis virus

2. According to the current CDC-recommended laboratory algorithm for diagnosing HIV infection in adults and children aged >18 months, what is the recommended initial screening test?

A) HIV RNA nucleic acid test (NAT)
B) HIV-1/HIV-2 antigen/antibody combination immunoassay
C) HIV-1/HIV-2 antibody differentiation immunoassay
D) HIV-1 p24 antigen assay only

3. A patient develops a severe diarrheal illness after consuming raw oysters. Stool cultures grow a Gram- negative, comma-shaped bacterium that is oxidase-positive and grows on thiosulfate-citrate-bile salts-sucrose (TCBS) agar, producing green colonies. The MOST likely virulence factor contributing to the watery diarrhea is:

A) Cholera toxin-like enterotoxin
B) Shiga toxin
C) Heat-labile toxin (LT)
D) Cytotoxin-associated gene A (CagA)

4. A microbiology laboratory is investigating an outbreak of bloodstream infections in a neonatal intensive care unit. Isolates of Staphylococcus epidermidis from multiple infants show indistinguishable banding patterns by pulsed-field gel electrophoresis (PFGE). This finding MOST strongly suggests:

A) Widespread antibiotic resistance within the S. epidermidis population.
B) Recent horizontal gene transfer of virulence factors.
C) A common source of infection and clonal spread of the organism.
D) Spontaneous mutations leading to increased pathogenicity.

5. A key virulence factor of Neisseria meningitidis that plays a major role in immune evasion and is the target for serogrouping is the:

A) Pili
B) Polysaccharide capsule
C) Lipooligosaccharide (LOS) endotoxin
D) IgA protease

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